Coding

Part:BBa_K4162001:Design

Designed by: Weiwen Chen   Group: iGEM22_Fudan   (2022-08-21)


StayGold = (n2)oxStayGold(c4)v2.0


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 638
    Illegal BsaI.rc site found at 658


Design Notes

codon-optimized


Source

The ybbO gene is reported to have transferred from an eukaryotic retinol dehydrogenase ancestor to the E. coli genome in the human intestine via horizontal gene transfer, as shown by sequence comparison and phylogenetic analysis.

References